Which of the following is the correct match of enzymes with their rule in genetic engineering?

DNA polymerase - cutting the DNA

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Restriction enzyme - cutting the DNA

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DNA ligase - helps in DNA replication

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Restriction enzyme - helps in DNA replication.

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Solution

The correct option is B

Restriction enzyme - cutting the DNA

Restriction enzyme cut DNA duplex at the specific location. They are obtained from bacteria which produces them to restrict foreign DNAs.
DNA polymerase helps in the polymerisation of DNA strand and DNA ligase joins two fragments of DNA.

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Q. Explain the mechanism of DNA replication with the help of a replication fork. What role does the enzyme DNA-ligase play in a DNA replication fork?

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Deoxyribonucleotides, buffer, E. coli, Taq DNA polymerase, primer, DNA probe, DNA cutting (restriction) enzymes, host cell

Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Match the following enzymes of DNA replication with their respective functions:

p. DNA polymerase i. Helps unzip the DNA double helix

q. DNA ligase ii. Seals nicks between Okazaki fragments

r. DNA helicase iii. Lays RNA primers to which polymerase can add nucleotides

s. Primase iv. Adds nucleotides to the free 3’ end of RNA primers

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Which of the following is the correct match of enzymes with their rule in genetic engineering?

The correct option is B Restriction enzyme - cutting the DNA Restriction enzyme cut DNA duplex at the specific location. They are obtained from bacteria which produces them to restrict foreign DNAs. DNA polymerase helps in the polymerisation of DNA strand and DNA ligase joins two fragments of DNA.