Which of the following is the method developed by Matthew Meselson and Franklin Stahl to separate heavy DNA with N $^{15}$ from DNA with N $^{14}$ , for providing evidence for semi-conservative replication of DNA?

Ion exchange chromatography

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Density gradient centrifugation

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Buoyant density centrifugation

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Gel filtration

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Isopycnic centrifugation

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Solution

The correct option is A Density gradient centrifugation
The method developed by Matthew Meselson and Franklin StahI to separate heavy DNA with N $^{15}$ from DNA with N $^{14}$ was density gradient centrifugation. it separates the DNA molecules in different layers according to their density. The sample is placed on a preformed gradient cesium chloride. Centrifugation separates the macromolecules in different bands of density gradient which can be collected as a pure fraction. Option B is correct answer.

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Q. Matthew Meselson and Franklin Stahl grew E. coli(doubling time is

20

min) in medium containing

^{15} N H_{4} C l

for many generations. Then the E. coli was transferred to medium containing

^{14} N H_{4} C l

. After

40

minutes, the cells were harvested and DNA was extracted and subjected to cesium chloride density gradient centrifugation. The proportion of light and hybrid DNA densities will be.

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Which of the following is the method developed by Matthew Meselson and Franklin Stahl to separate heavy DNA with N15 from DNA with N14, for providing evidence for semi-conservative replication of DNA?

Which of the following is the method developed by Matthew Meselson and Franklin Stahl to separate heavy DNA with N $^{15}$ from DNA with N $^{14}$ , for providing evidence for semi-conservative replication of DNA?