Question

Which of the following is true about insertional inactivation of the β-galactosidase coding sequence?

• A. The transformants exhibit blue coloured
• B. The non-transformants exhibit blue coloured
• C. The transformants do not exhibit any colour
• D. The Non-transformants do not exhibit any colour

Answer 1

The correct option is C

The transformants do not exhibit any colour

If the plasmid in the bacterium does not have an insert, the presence of chromogenic substance gives blue coloured colonies. Presence of insert results into insertional inactivation of β-galactosidase and therefore the colonies do not produce colour.