3130
Question
Which of the following mediums were used to culture bacteria by Meselson and Stahl in their experiments?
Which of the following mediums were used to culture bacteria by Meselson and Stahl in their experiments?
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Solution
The correct option is A 14NH4Cl and 15NH4Cl
Meselson and Stahl utilised the technique of density gradient centrifugation in their experiments. They grew E.coli in a medium containing 15NH4Cl. Consequently, all the DNA molecules got 15N incorporated into them. After that, the cells were transferred into a medium containing normal NH4Cl. The cells were isolated at different time intervals, their double stranded DNA was extracted and separated in CsCl density gradient.
Meselson and Stahl utilised the technique of density gradient centrifugation in their experiments. They grew E.coli in a medium containing 15NH4Cl. Consequently, all the DNA molecules got 15N incorporated into them. After that, the cells were transferred into a medium containing normal NH4Cl. The cells were isolated at different time intervals, their double stranded DNA was extracted and separated in CsCl density gradient.
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