Question

Which of the following options correctly explains the sequence of processes in PCR?

• A.
  Primer annealing → DNA synthesis → Denaturation
• B.
  Primer annealing → Denaturation → DNA synthesis
• C.
  Denaturation → Primer annealing → DNA synthesis
• D.
  Denaturation → DNA synthesis → Primer annealing

Answer 1

The correct option is C

Denaturation → Primer annealing → DNA synthesis
Denaturing – when the double-stranded template DNA is heated to separate it into two single strands.

Annealing – when the temperature is lowered to enable the DNA primers to attach to the template DNA.

Extending – when the temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.
Steps involved are as follows:
Step 1 - Denaturation. The solution contained in the tube is heated to at least 94 degree Celcius.

Step 2-Primer design.

Step 3-Enzyme selection.

Step 4-Thermal cycling.

Step 5-Amplicon analysis.

So, the option is C