The correct option is D Rhizobium
Nodule formation is observed in the roots of leguminous plants which have symbiotic relationship with nitrogen fixing bacteria like Rhizobium. Rhizobium are present in the roots of several legumes such as alfalfa, sweet clover, sweet pea, lentils, etc.
Azotobacter, Anabaena and Nostoc are the free living nitrogen fixation organisms.