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Question
Which of the following plasmid does not show resistant to ampicillin?
Which of the following plasmid does not show resistant to ampicillin?
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Solution
The correct option is D None of the above
pBR322 carries two sets of antibiotic resistance genes. pBR327 plasmid is a higher copy number plasmid and was constructed by removing a 1089 bp segment from pBR322. pUC8 plasmid is derived from pBR322, remaining only the replication origin and the ampR gene. Either ampicillin or tetracycline resistance can be used as a selectable marker for cells containing all the three plasmids, and each marker gene includes unique restriction sites that can be used in cloning experiments.Thus, the correct answer is option D.
pBR322 carries two sets of antibiotic resistance genes. pBR327 plasmid is a higher copy number plasmid and was constructed by removing a 1089 bp segment from pBR322. pUC8 plasmid is derived from pBR322, remaining only the replication origin and the ampR gene. Either ampicillin or tetracycline resistance can be used as a selectable marker for cells containing all the three plasmids, and each marker gene includes unique restriction sites that can be used in cloning experiments.
Thus, the correct answer is option D.
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