1
Question
Which of the following process(es) is not involved as a step in DNA fingerprinting?
Which of the following process(es) is not involved as a step in DNA fingerprinting?
Open in App
Solution
The correct option is C DNA transcription
DNA fingerprinting is a laboratory technique which relies on the repetitive sequences of DNA of a person to determine his/ her unique identity. It involves the following steps:-
- DNA extraction: Since the DNA content of every cell is the same, hence it can be extracted from the samples available like blood, saliva, semen, hair follicle etc.
- Polymerase Chain reaction: Amplification of DNA is done by Polymerase Chain reaction to increase its quantity.
- Restriction digestion: Cleavage of DNA is done by using restriction enzymes which yields several DNA fragments.
- Gel electrophoresis: These DNA fragments are then transferred to Agarose gel for gel electrophoresis. This leads to separation of DNA fragments of varying sizes which then get arranged according to their molecular weight.
- Southern Blotting: The separated DNA fragments present in the gel are transferred to a nylon or nitrocellulose membrane, the process called southern blotting. Treatments are given so as to get single strands out of the double stranded DNA that got transferred onto the membrane.
- Hybridisation: To detect the presence of repetitive site locus, a single stranded radioactive probe or fluorescent probe is used which is complementary to the satellite sequence. The probe is then allowed to hybridise with the DNA on the membrane.
- Autoradiography: Lastly, the nylon or nitrocellulose membrane is exposed to X-rays. Specific bands appear on the X ray film corresponding to the areas of hybridisation.
DNA transcription is the process by which the information from one of the strands of DNA is copied to an mRNA molecule. It is not a step in DNA fingerprinting
DNA fingerprinting is a laboratory technique which relies on the repetitive sequences of DNA of a person to determine his/ her unique identity. It involves the following steps:-
- DNA extraction: Since the DNA content of every cell is the same, hence it can be extracted from the samples available like blood, saliva, semen, hair follicle etc.
- Polymerase Chain reaction: Amplification of DNA is done by Polymerase Chain reaction to increase its quantity.
- Restriction digestion: Cleavage of DNA is done by using restriction enzymes which yields several DNA fragments.
- Gel electrophoresis: These DNA fragments are then transferred to Agarose gel for gel electrophoresis. This leads to separation of DNA fragments of varying sizes which then get arranged according to their molecular weight.
- Southern Blotting: The separated DNA fragments present in the gel are transferred to a nylon or nitrocellulose membrane, the process called southern blotting. Treatments are given so as to get single strands out of the double stranded DNA that got transferred onto the membrane.
- Hybridisation: To detect the presence of repetitive site locus, a single stranded radioactive probe or fluorescent probe is used which is complementary to the satellite sequence. The probe is then allowed to hybridise with the DNA on the membrane.
- Autoradiography: Lastly, the nylon or nitrocellulose membrane is exposed to X-rays. Specific bands appear on the X ray film corresponding to the areas of hybridisation.
Suggest Corrections
1
Similar questions
View More
Join BYJU'S Learning Program
Explore more
Join BYJU'S Learning Program
