Which of the following properties of DNA polymerase necessitates the requirement of RNA primer in DNA replication?

It can add a nucleotide only to a 3' end

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It gets denatured at high temperatures

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None of the above

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It can add a nucleotide only to a 5' end

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Solution

The correct option is A
It can add a nucleotide only to a 3' end

DNA polymerase can add a nucleotide only to a free 3'-end. Thus, to start the replication reaction a free 3'-end is provided by the RNA primer as there is no free 3-end available for adding the first nucleotide. RNA primer is a short segment of RNA synthesized at the 5' end of DNA. It gives free 3' end for the polymerase to elongate the DNA.

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Similar questions

Which of the following properites of DNA polymerise necessitates the requirement of RNA primer in DNA replication?

Q. How many of the following statements are correct ?

A. DNA dependent DNA polymerase requires DNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

B. DNA dependent DNA polymerase requires RNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

C. During replication, unwinding of DNA double helix forms a fork-like structure called replication fork.

D. During transcription, unwinding of the DNA double helix by DNA dependent RNA polymerase forms a transcription bubble.

E. During transcription, unwinding of DNA double helix by DNA dependent RNA polymerase forms a transcription fork.

Match the following enzymes of DNA replication with their respective functions:

p. DNA polymerase i. Helps unzip the DNA double helix

q. DNA ligase ii. Seals nicks between Okazaki fragments

r. DNA helicase iii. Lays RNA primers to which polymerase can add nucleotides

s. Primase iv. Adds nucleotides to the free 3’ end of RNA primers

Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Q. The type of RNA polymerase controlling DNA replication are

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Process of Translation

Standard XII Biology

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