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Question

Which of the following reagents is used to estimate the number of hydroxyl groups in reducing sugars?

A
PCl5
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B
HIO4
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C
(CH3CO)2O
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D
HI/Red P
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Solution

The correct option is C (CH3CO)2O
The quantitative measurement of free hydroxyl groups in partially substituted sugar is done by acetylation. By measuring the excess of acetic anhydride added to the sample, the number of free hydroxyl groups are estimated.

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