Which of the following required for binding protein with nucleic acid?

Nickel

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Iron

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Cobalt

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Calcium

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Solution

The correct option is D Calcium
Many enzymes acting on DNA require Mg²⁺ ions not only for catalysis but also to bind DNA. Ca²⁺ acts as a substitute for Mg²⁺ to promote DNA binding. The Sfi-1 endonuclease requires divalent metal ions to bind DNA but, in contrast to many systems where Ca²⁺ mimics Mg²⁺, Ca²⁺ causes Sfi-1 to bind DNA almost irreversibly. Hence the correct option is D.

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