619
Question
Which of the following restrictions enzymes produces blunt ends?
Which of the following restrictions enzymes produces blunt ends?
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Solution
The correct option is A Eco RV
Restriction enzymes are the special enzymes which can recognise sites in the DNA sequences and make a cut at these sites. Restriction enzymes cut the DNA in middle leaving blunt ends on the DNA or staggered cuts are made that leave sticky ends with 5’or 3’ overhangs. These sticky overhangs can reanneal spontaneously by complementary base pairing. The restriction site of Eco RV is GAT_ATC and it produces blunt ends. The adaptor molecules are used to create sticky ends at the fragments which are cleaved with the help of this enzyme.
Thus, the correct answer is option D.
Restriction enzymes are the special enzymes which can recognise sites in the DNA sequences and make a cut at these sites. Restriction enzymes cut the DNA in middle leaving blunt ends on the DNA or staggered cuts are made that leave sticky ends with 5’or 3’ overhangs. These sticky overhangs can reanneal spontaneously by complementary base pairing. The restriction site of Eco RV is GAT_ATC and it produces blunt ends. The adaptor molecules are used to create sticky ends at the fragments which are cleaved with the help of this enzyme.
Thus, the correct answer is option D.
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