Question

Which of the following(s) is/are reqiured in PCR?

• A. Nucleotides
• B. Buffer
• C. DNA polymerase
• D. Primer

Answer 1

Answer: (A), (B), (C), (D)

Primer

Nucleotides, DNA polymerase, primer, buffer, all are needed for PCR. In PCR the template DNA is amplified. The primer attaches to the template DNA, after this the DNA polymerase extends the DNA with the use of nucleotides. The buffer maintains the pH of the reaction to keep the enzymes active.