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Question

Which of the following sequence is most likely to be a restriction enzyme recognization site ?

A
CGGCTT
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B
GGGCCA
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C
GTAATG
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D
GTCGAC
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Solution

The correct option is D GTCGAC
  • Restriction enzymes are found in bacteria (and other prokaryotes). They recognize and bind to specific sequences of DNA, called restriction sites. Each restriction enzyme recognizes just one or a few restriction sites.
  • A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule wherein reading in a certain direction on one strand matches the sequence reading in the same direction on the complementary strand.
  • most restriction enzymes recognize a "perfect" palindrome, Hence GTCGAC(the complimentary sequence is CAGCTG) sequence is most likely to be a restriction enzyme recognization site,
  • So, the correct answer is "GTCGAC'.

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