Question

Which of the following shows correct chronological order of the events occurring during callus culture?

• A. Callus $\to$ Cell division $\to$ Explant $\to$ Addition of cytokinin $\to$ Acquire meristematic property
• B. Explant $\to$ Callus $\to$ Cell division $\to$ Addition of cytokinin $\to$ Cells acquire meristematic property
• C. Explant $\to$ Cell division $\to$ Callus $\to$ Addition of cytokinin $\to$ Cells acquire meristematic property
• D. Callus $\to$ Explant $\to$ Cell division $\to$ Addition of cytokinin $\to$ Cells acquire meristematic property

Answer 1

Answer: (C)

Explant $\to$ Cell division $\to$ Callus $\to$ Addition of cytokinin $\to$ Cells acquire meristematic property

During cell callus culture, a plant tissue or an explant is taken which is introduced into the medium, which then starts rapid cell division. It then forms callus. Further addition of required phytohormones in required amounts will result in the meristematic activity.