Which of the following shows the correct sequence of steps in a polymerase chain reaction (PCR)?
A
Denaturation, extension and annealing
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B
Annealing, extension and denaturation
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C
Extension, denaturation and annealing
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D
Denaturation, annealing and extension
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Solution
The correct option is D Denaturation, annealing and extension Polymerase chain reaction (PCR) is a method for the amplification of specific DNA sequences.