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Question 4

Which of the following stain is not used for staining chromosomes?

(a) Basic fuschsin (b) Safranin

(c) Methylene blue (d) Carmine

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Solution

(b) Safranin It is used as a counterstain in gram staining and endospore staining. It can also be used for detection of cartilage, mucin and mast cell granule.

Carmine The basic dye, is used to stain nucleic acid and chromosomes, which possess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.

Basic Fuschin It involves in staining of human chromosomes elastic fibres, cardiac or skeletal muscle tissue.

Methylene blue it is used to stain nuclei, Golgi bodies and pectic substances.


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