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Question

Which of the following stain is used to observe bacteria under the microscope?

A
Malachite green
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B
Methylene blue
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C
Crystal violet
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D
None of the above
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Solution

The correct option is C Crystal violet
The Gram staining is the common method which is used for staining and observing the bacteria. The method includes the components are crystal violet, Gram's iodine, alcohol and safranin. The stain is taken up by the bacterial cell wall due to the presence of peptidoglycan. The Gram-positive bacteria are stained with crystal violet and appear purple under the microscope. The Gram-negative bacteria are stained with safranin and appear pink.
Thus, the correct answer is option C.

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