Question

Which of the following statement can be related to UV spectroscopy?

• A. The comparative study of absorbance and transmittance of reference and sample is done.
• B. It follows lambert beer law.
• C. It cannot be used for cell count.
• D. Both A and B.

Answer 1

The correct option is D Both A and B.

The Beer-Lambert law states that the quantity of light absorbed by a sample is directly proportional to its concentration and the path length of light that passes through the sample. This law forms the basis of UV spectroscopy. Transmittance (T) is defined as the fraction of light that passes through the sample. It is related to absorption by the expression

Absorbance(A)= --logT=--logIt/Io, where It is the amount of light transmitted and Io is the amount of light incident on the cuvette sample. Hence it can be used for cell counting in a sample by determining the transmittance.

So, the correct answer is 'Both A and B.'