Question

Which of the following statement is/are incorrect regarding the plasmid pSC101?

i. It has an EcoRI restriction site
ii. It has a tetracycline resistance gene
iii. Restriction site for EcoRI is located in tetracycline resistance gene
iv. It has a kanamycin resistance gene

• A. i
• B. ii and iii
• C. iii and iv
• D. vi

Answer 1

The correct option is C iii and iv

Plasmid pSC101 has only one EcoRI recognition site. It has a tetracycline resistance gene or TC gene and the EcoRI recognition site is not present within the tetracycline resistant gene. It does not possess a kanamycin resistance gene.