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Question

Which of the following statements are correct?
a. Callus is the disorganised mass of cells formed under in vitro condition.
b. Totipotency is the ability of a plant organ to regenerate an entire organism when cultured in a suitable medium.
c. Embryoid is the embryo-like structure formed under in vitro condition which has the potentiality to develop into a plantlet.
d. Explant is the excised portion of plant tissue or organ which is used in tissue culture study.
Select the correct answer using the codes given Codes.

A
1,2 and 4
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B
1,3 and 4
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C
1,2 and 3
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D
2,3 and 4
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Solution

The correct option is A 1,2 and 4
  • Callus is defined as a group of cells derived from competent source tissue that is cultured under in vitro conditions to form an undifferentiated mass of cells.
  • Totipotency denotes the ability of a cell to generate an entire organism. For instance, if separated, each blastomere from a mouse 2-cell embryo is capable of developing into a complete organism.
  • An explant is a cell, organ, or piece of tissue which has been transferred from animals or plants to a nutrient medium.
  • Embryoid bodies are aggregates of pluripotent cells that are induced to differentiate by a combination of a change in culture medium (removal of factors that support pluripotency) and allowing the cells to interact in three-dimensional structures.
So, the correct option is 'A'.

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