1
Question
Which of the following statements are correct with respect to a bioreactor?
i. It can process large volumes of culture
ii. It provides optimum temperature and pH
iii. It is a compact thermal cycler
Which of the following statements are correct with respect to a bioreactor?
i. It can process large volumes of culture
ii. It provides optimum temperature and pH
iii. It is a compact thermal cycler
Open in App
Solution
The correct option is A i and ii
Bioreactors are large vessels which are used to grow large volumes of culture (containing microbial, plant or mammalian cells) for obtaining the desired gene product. These are usually provided with systems for temperature and pH control, sampling ports, inlets for addition of nutrient medium and inoculum, steam inlets for sterilisation and outlets for product extraction.
With the help of the temperature and pH control systems, bioreactors provide optimal growth conditions for the cells.
Addition of buffers helps to regulate the pH levels in a bioreactor. Generally, pH ranging between 6-8 is needed for the biosynthesis of desired gene products.
The ongoing metabolic reactions inside cells generate a lot of heat. This is controlled by cooling jackets that externally envelope the bioreactor. The space between the jacket and the vessel is filled with water that absorbs excess heat and maintains optimum temperature.
A compact thermal cycler is an apparatus that can alter the reaction temperature over a wide range at appropriate intervals as per programming. It is generally used for amplifying DNA segments in the process of polymerase chain reaction (PCR) where the reaction condition has to get frequent alterations.
Bioreactors do not act as thermal cyclers.
Bioreactors are large vessels which are used to grow large volumes of culture (containing microbial, plant or mammalian cells) for obtaining the desired gene product. These are usually provided with systems for temperature and pH control, sampling ports, inlets for addition of nutrient medium and inoculum, steam inlets for sterilisation and outlets for product extraction.
With the help of the temperature and pH control systems, bioreactors provide optimal growth conditions for the cells.
Addition of buffers helps to regulate the pH levels in a bioreactor. Generally, pH ranging between 6-8 is needed for the biosynthesis of desired gene products.
The ongoing metabolic reactions inside cells generate a lot of heat. This is controlled by cooling jackets that externally envelope the bioreactor. The space between the jacket and the vessel is filled with water that absorbs excess heat and maintains optimum temperature.
A compact thermal cycler is an apparatus that can alter the reaction temperature over a wide range at appropriate intervals as per programming. It is generally used for amplifying DNA segments in the process of polymerase chain reaction (PCR) where the reaction condition has to get frequent alterations.
Bioreactors do not act as thermal cyclers.
Suggest Corrections
0
Similar questions
View More
Join BYJU'S Learning Program
Explore more
Join BYJU'S Learning Program
