Question

Which of the following statements can be related to ELISA?

• A. It uses complement mediated cell lysis.
• B. It uses radiolabelled second antibody.
• C. It involves addition of substrate which is converted into colored end product.
• D. It requires RBC's.

Answer 1

The correct option is C It involves addition of substrate which is converted into colored end product.

• ELISA(enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones.
• In ELISA, antigens from the sample are attached to a surface. Then, a matching antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and in the final step, a substance containing the enzyme's substrate is added.
• The reaction produces a detectable signal, a colour change. Hence, the statements can be related to ELISA is that It involves the addition of substrate which is converted into the coloured end product.

So, the correct answer is 'It involves the addition of substrate which is converted into coloured end product'.