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Question
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
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Solution
The correct option is B Extension of primer end on the template DNA
Polymerase Chain Reaction:
Polymerase chain reaction or PCR is a reaction carried in vitro to amplify a specific sequence of DNA or gene of interest by creating its multiple copies.
Denaturation of template DNA:
Denaturation involves the separation of the two strands of double stranded template DNA by the application of heat at 94−95∘C.
Annealing of primers to template DNA:
Annealing involves hybridisation of the primers with specific sequences of each of the strands of the template DNA. This process is carried out at 50−56∘C.
Extension of primer end on the template DNA:
In extension, the Taq DNA polymerase synthesises DNA by adding dNTPs to the free ends of the primers - forward and reverse. The extension of DNA is carried out at 72∘C. This helps in synthesising complementary strands.
Final answer
c. Extension of primer end on the template DNA
Polymerase Chain Reaction:
Polymerase chain reaction or PCR is a reaction carried in vitro to amplify a specific sequence of DNA or gene of interest by creating its multiple copies.
Denaturation of template DNA:
Denaturation involves the separation of the two strands of double stranded template DNA by the application of heat at 94−95∘C.
Annealing of primers to template DNA:
Annealing involves hybridisation of the primers with specific sequences of each of the strands of the template DNA. This process is carried out at 50−56∘C.
Extension of primer end on the template DNA:
In extension, the Taq DNA polymerase synthesises DNA by adding dNTPs to the free ends of the primers - forward and reverse. The extension of DNA is carried out at 72∘C. This helps in synthesising complementary strands.
Final answer
c. Extension of primer end on the template DNA
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