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Question

Which of the following technique can be used for gene transfer in the formation of transgenic plants?

A
Microinjection
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B
Electroporation
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C
Centrifugation
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D
Both A and B
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Solution

The correct option is C Both A and B
Gene transfer includes the transfer of the foreign gene of interest into the host plant cell. Microinjection includes direct injection of DNA is into plant protoplasts or cells using fine-tipped glass needle. Electroporation is the process in which high voltage pulse is applied to cells and thus making transient pores in the plasma membrane. These pores facilitate the uptake of foreign DNA.
Thus, the correct answer is option D.

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