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Question
Which of the following technique is demonstrated in the above image.
Which of the following technique is demonstrated in the above image.
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Solution
The correct option is C Sandwich ELISA
The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. In sandwich ELISA, the antibody is coated to the wells of the ELISA plate. If there is specific (primary) antibody, they will bind to the antigen which is added. The secondary antibody attached to the enzyme binds to the primary antibody. The chromogenic substrate is added which is acted upon by the enzyme and the colour is produced which can be read and detected by the ELISA reader. Thus, the correct answer is option C.
The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. In sandwich ELISA, the antibody is coated to the wells of the ELISA plate. If there is specific (primary) antibody, they will bind to the antigen which is added. The secondary antibody attached to the enzyme binds to the primary antibody. The chromogenic substrate is added which is acted upon by the enzyme and the colour is produced which can be read and detected by the ELISA reader.
Thus, the correct answer is option C.
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