Question

Which of the following technique use capture and detecting antibody?

• A. Direct ELISA
• B. Indirect ELISA
• C. Sandwich ELISA
• D. All of the above

Answer 1

The correct option is C Sandwich ELISA

Sandwich ELISA is a less common variant of ELISA, but is highly efficient in sample antigen detection. It quantify antigens between two layers of antibodies i.e., capture and detection antibody. The antigen to be measured must contain at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich.

So, the correct answer is option C.