Question

Which of the following techniques are used in analysing restriction fragment length polymorphism (RFLP)?

(A) Electrophoresis (B) Electroporation
(C) Methylation (D) Restriction digestion

• A. A and C
• B. C and D
• C. A and D
• D. B and D

Answer 1

The correct option is C A and D

Sequence polymorphisms are minor sequence differences (a single base-pair changes) between individuals. Restriction enzymes can digest the whole genome into DNA fragments of specific length based on the location of restriction sites in the genome. Sequence polymorphism affects recognition sites for restriction enzymes which in turn causes variation in the size of DNA fragments produced by digestion with a particular restriction enzyme. These variations are restriction fragment length polymorphisms (RFLPs) which are detected by electrophoresis. For the purpose, restriction enzyme digested DNA samples are sorted by gel electrophoresis according to their size; option C is correct. Electroporation is the method of direct gene transfer by applying a pulse of high voltage applied to protoplasts/cells/ tissues to make transient pores in the plasma membrane which serve to uptake the foreign DNA which makes option D wrong. DNA methylase enzyme carries out methylation of DNA to protect own DNA in bacteria from restriction digestion which makes options A and B wrong.

So, the correct answer is option C.