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Question

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

A
Reverse transcriptase-production of.cDNA from mRNA
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B
DNA polymerase-used in polymerase chain reaction to amplify section of DNA
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C
DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments
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D
Restriction enzyme-production of RFLPs
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Solution

The correct option is C DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments
Restriction enzymes cut DNA creating sticky ends of restriction fragments. While DNA ligase joins these sticky ends to form recombinant DNA.
So, the correct option is 'DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments'.

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