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Question
Which one among the following is just a cloning plasmid and not a expression plasmid?
Which one among the following is just a cloning plasmid and not a expression plasmid?
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Solution
The correct option is D pUC19
- Cloning plasmids are the plasmids that serve to carry the foreign DNA segment into the host cell while expression plasmids carry expression signals also to ensure maximum expression of the foreign gene.
- pBAD-18-Cam, pBCSK and pET are expression plasmids carrying strong promoter and termination codons to ensure maximum gene expression.
- pUC19 is a cloning vector-only and does not carry the sequences to facilitate gene expression.
Thus, the correct answer is option C.
- Cloning plasmids are the plasmids that serve to carry the foreign DNA segment into the host cell while expression plasmids carry expression signals also to ensure maximum expression of the foreign gene.
- pBAD-18-Cam, pBCSK and pET are expression plasmids carrying strong promoter and termination codons to ensure maximum gene expression.
- pUC19 is a cloning vector-only and does not carry the sequences to facilitate gene expression.
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