Which one among the following is needed for tissue culture?
A
Kinetin
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B
Coumarine
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C
Trypsin
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D
Caffine
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Solution
The correct option is A
Kinetin
In order to initiate plant tissue culture, one needs; explants, suitable culture/growth media, aseptic conditions to curb growth of microorganisms, water, growth regulators (auxins and cytokinins), and frequent sub culturing to avoid accumulation of waste metabolite and enhance nutrition.
Cytokinins- these are purine derivatives that support cell division. The two main types of cytokinins used in tissue culture are benzylaminopurine (BAP) and kinetine.