Question

Which one is a true statement regarding DNA polymerase used in PCR?

• A. It is isolated from a virus.
• B. It remains active at high temperature.
• C. It is used to ligate introduced DNA in recipient cells.
• D. It serves as a selectable marker.

Answer 1

Answer: (B)

It remains active at high temperature.

The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75${}^o$C and the ability to retain that activity after prolonged incubation at even higher temperatures (95${}^o$C). The first thermostable DNA polymerase to be widely used for PCR is Taq DNA Polymerase.