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Question
Which one of the following can be visualized under fluorescent microscope?
Which one of the following can be visualized under fluorescent microscope?
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Solution
The correct option is D Both A and B
Fluorescent microscope is a modification of ultraviolet microscope, which was made by Coons in 1945. The instrument uses long wave ultraviolet rays for illumination. It has complementary filters, which allow the viewer to observe directly with the eyes. The microscope is useful in detecting those components which show autofluorescence, e.g., chlorophylls, collagen fibrils, actin filaments, vitamin A, etc. Others can be made fluorescent by coating with fluorochrome dyes like acridine orange and coriphosphine and minerals, eg., proteins, lipids, starch, glycogen, cellulose, etc. The light emitted by them is red, orange, yellow, or green against dark field. It is called as secondary fluorescence. The technique is also useful in diagnosis of viruses, bacteria and protozoans. Immunofluorescent antibody labelling techniques is used in diagnosis of specific molecules like antigens and antibodies. it involves conjugating specific fluorochromes with specific antibodies.
Fluorescent microscope is a modification of ultraviolet microscope, which was made by Coons in 1945. The instrument uses long wave ultraviolet rays for illumination. It has complementary filters, which allow the viewer to observe directly with the eyes. The microscope is useful in detecting those components which show autofluorescence, e.g., chlorophylls, collagen fibrils, actin filaments, vitamin A, etc. Others can be made fluorescent by coating with fluorochrome dyes like acridine orange and coriphosphine and minerals, eg., proteins, lipids, starch, glycogen, cellulose, etc. The light emitted by them is red, orange, yellow, or green against dark field. It is called as secondary fluorescence. The technique is also useful in diagnosis of viruses, bacteria and protozoans. Immunofluorescent antibody labelling techniques is used in diagnosis of specific molecules like antigens and antibodies. it involves conjugating specific fluorochromes with specific antibodies.
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