Question

Which one of the following characteristics is generally not preferred for a cloning vector?

• A. An origin of replication
• B. An antibiotic resistance marker
• C. Multiple restriction sites
• D. A high copy number

Answer 1

Answer: (C)

Multiple restriction sites

Cloning vectors is a small piece of DNA into which foreign DNA fragments can be inserted for cloning purpose . Cloning vectors are used as functional origin of replication, selectable marker and gives a high number of copies. This may be a multiple cloning site which contains a unique restriction sites.

So, the correct answer is 'Option C' .