Question

Which one of the following enzyme combinations yields protoplasts satisfactorily?

• A. Ligninase-cellulose-lipase
• B. Cellulase-lipids-hemicellulase
• C. Cellulase-hemicellulase-ligninase
• D. Pectinase-cellulose-Hemicellulase

Answer 1

The correct option is D Pectinase-cellulose-Hemicellulase

Ligninase helps in breaking down of the lignin. Cellulase helps in digestion of the cellulose of plant cell wall. Lipase helps in the breakdown of fat. Hemicellulase helps in the digestion of the cell wall. Pectinase helps in the breakdown of pectin, which acts as to hold the cells together. On removing the cell wall from the cell the protoplast gets into a spherical shape and we can yeild the protoplast satisfactorily.

So, the correct option is 'D'.