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Question
Which one of the following enzymes is not necessary for obtaining protoplasts for their somatic hybridization?
Which one of the following enzymes is not necessary for obtaining protoplasts for their somatic hybridization?
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Solution
The correct option is B Primase
Isolation of protoplasts is readily achieved by treating cells/tissues with a suitable mixture of cell wall degrading enzymes. Usually, a mixture of pectinase or macerozyme (0.1-1.9%) and cellulose (1-2%) is appropriate for most plant materials. Hemicellulase may be necessary for some tissues.
Isolation of protoplasts is readily achieved by treating cells/tissues with a suitable mixture of cell wall degrading enzymes. Usually, a mixture of pectinase or macerozyme (0.1-1.9%) and cellulose (1-2%) is appropriate for most plant materials. Hemicellulase may be necessary for some tissues.
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