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Question

Which one of the following equations suggests that O2 released during photosynthesis comes from water?

A
6CO182+12H2O6O182+C6H12O6+6H2O18
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B
6CO2+12H2O186O2+C6H12O6+6H2O18
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C
6CO182+12H2O6O182+C6H12O6+6H2O
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D
6CO2+12H2O186O182+C6H12O6+6H2O
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Solution

The correct option is D 6CO2+12H2O186O182+C6H12O6+6H2O
Ruben and Kamen (1941), while working on chlorella (unicellular green alga ) found that oxygen liberated during photosynthesis comes from water.
(i) When normal H2O radioactive CO2(i.e.CO182) were used, normal O2 is evolved.

nCO182+nH2OLight−−−−−−Chlorophyll(CH2O18)n+nO2(normal)+H2O

(ii) When normal CO2 radioactive H2O(i.e.H2O18) were used, radioactive O2 (i.e.O182) is evolved.

nCO2+nH2O18(CH2O)n+O182(radioactiveoxygen)+H2O
So the correct option is 'D'.

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