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Question

Which one of the following hydrolyzes internal phosphodiester bonds in a polynucleotide chain:

A
Lipase
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B
Protease
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C
Exonuclease
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D
Endonuclease
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Solution

The correct option is D Endonuclease
Nuclease is an enzyme capable of cleaving the phosphodiester bonds between monomeric units of nucleic acids.
Endonucleases are enzymes that hydrolyze internal phosphodiester bonds in a polynucleotide chain.
Exonucleases are enzymes that work by cleaving nucleotides one at a time from the ends of a polynucleotide chain.
lipases are enzymes that catalyze the hydrolysis of fats (lipids).
Proteases are enzymes that catalyze the hydrolysis of proteins and peptides.

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