Question

Which one of the following is a recognition site for restriction enzyme Bam HI?

• A. $5^{\prime }$- GAATTC- $3^{\prime }{3}^{\prime }$- CTTAAG-$5^{\prime }$
• B. $5^{\prime }$- GGATCC- $3^{\prime }{5}^{\prime }$- CCATGG-$3^{\prime }$
• C. $5^{\prime }$- GGATCC-$3^{\prime }{3}^{\prime }$-CCTAGG-$5^{\prime }$
• D. $5^{\prime }$-GAATTC-$3^{\prime }{5}^{\prime }$-GTTAAC-$3^{\prime }$

Answer 1

The correct option is C $5^{\prime }$- GGATCC-$3^{\prime }{3}^{\prime }$-CCTAGG-$5^{\prime }$

Bam H1 is a type of restriction endonuclease enzyme that binds to the nonspecific DNA and recognizes the sequence of 6bp. It produces sticky end when making a nick at the base of guanosine.