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Discovery of Restriction Enzyme

Which one of ...

Question

Which one of the following is not a restriction endonuclease?

A

Eco-RI

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B

Hind-III

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C

Pst-I

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D

DNase-I

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Solution

The correct option is D
DNase-I

DNase-I is an endonuclease but do not cut the DNA at a specific nucleotide sequence. A restriction endonuclease on the other hand, finds a specific site.

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Q. Which among the following restriction endonucleases will produce blunt ends?
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\frac{A A G C T T}{T T C G A A}

Which of the following sequence contained in a double stranded DNA is cut by Hind

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Q. In nomenclature of REN (restriction endo - nuclease)

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Q. Which of the following statements are correct?
(i) Restriction enzymes may cut the DNA strand a little away from the centre of the palindromic sequence, but between the same two bases on the opposite strands.
(ii) Hind ll always cuts DNA molecules at a particular site by recognising a specific sequence of six base pairs.
(iii) DNA is a positively charged molecule.

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