Question

Which one of the following is not essential for polymerase chain reaction (PCR)?

• A. Restriction enzyme
• B. Denaturation of DNA
• C. Primers
• D. DNA polymerase

Answer 1

The correct option is A Restriction enzyme

Polymerase chain reaction (PCR) is a technique used to amplify a single copy of desired segment of DNA up to several orders of magnitude. The process requires thermostable DNA polymerase (Taq polymerase), Specifically designed DNA primers and nucleotides. Each cycle consists of three steps, denaturation, annealing and extension.

Thus, the correct answer is option A.