The correct option is C They regulate opening of valves.
Both the tricuspid and mitral valves are attached by means of thin fibrous threads to the wall of the ventricles. The function of these threads, called the chordae tendineae, is to keep the valve flaps from flipping up into the atria when the ventricles contract and thus causing a backflow of blood. The chordae tendinae do not regulate the opening of tricuspid and bicuspid valves.