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External Factors Affecting the Rate of Transpiration.

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Question

Which one of the following is the correct sequence to prepare a stained temporary mount of an onion peel?

A

(i) take out onion peel (ii) keep the peel on the slide (iii) add a few drop of glycerine on it (iv) add few drops of safranin stain (v) cover it up with a coverslip

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B

(i) take out onion peel (ii) keep the peel in water in a petri dish (iii) add a few drops of safranin stain and transfer to a slide (iv) add a drop of glycerine (v) cover it up with a coverslip

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C

(i) take out onion peel (ii) Keep it on a slide and add safranin stain (iii) transfer it to water in a petri dish (iv) remove water and add glycerine (v) cover it up with a coverslip

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D

(i) take out onion peel (ii) cover it up with a coverslip (iii) on a petri dish, add water to clean (iv) add a drop of glycerine (v) add a few drops of safranin stain

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Solution

The correct option is B
(i) take out onion peel (ii) keep the peel in water in a petri dish (iii) add a few drops of safranin stain and transfer to a slide (iv) add a drop of glycerine (v) cover it up with a coverslip

(i) take out onion peel

(ii) keep the peel in water in a petri dish

(iii) add a few drops of safranin stain and transfer to a slide

(iv) add a drop of glycerine

(v) cover it up with a coverslip

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