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Question

Which one of the following is the correct set of three precautions for setting up the experiment to demonstrate that carbon dioxide is evolved during respiration?
(1) Air tight set up; delivery tube dips in water in beaker; flask has seeds which have just germinated.
(2) Thread holding KOH test tube; air tight flask; delivery tube above surface of water in the beaker.
(3) Germinated seeds under water in the flask; experimental set up not air tight; delivery tube above water level.
(4) Delivery tube touching bottom of water; KOH test tube held by a thick wire; seeds covered by water.

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Solution

The precautions which should be taken -
Air tight set up - an air tight set up is required, so that the CO2 produced during respiration does not escape out
Delivery tube dips in water in beaker - the delivery tube needs to touch the water, so that the water can rise in the tube when partial vacuum is created in the flask.
Flask has seeds which have just germinated - germinated seeds will produce CO2 during the experiment which is an important requirement for this experiment.

Hence, the correct answer is option 1.

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