Which one of the following sequence represents m-RNA coded from a DNA segment with base pairs as 5' GAGCGCACA 3', 3' CTCGCGTGT 5'?
A
GAGCGCACA
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B
CUCCGCUGU
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C
CTCGCGTGT
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D
CUCCGCUCC
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E
CUCGUGUGU
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Solution
The correct option is A GAGCGCACA The DNA strand that serves as the template in RNA synthesis during transcription is called anticoding or antisense strand, while the other strand is called coding or sense strand as its base sequence, is same as that of newly synthesized mRNA. The coding strand for a gene may be located in either strand of a given chromosome (5’ to 3’ strand or 3’ to 5’ strand). Thus, if the 5' GAGCGCACA 3' strand is a template; the mRNA sequence is 3' CTCGCGTGT 5' while if 3' CTCGCGTGT 5' is a template; the mRNA sequence is 5' GAGCGCACA 3'. Presence of thymidine in option C makes it incorrect as RNA has uracil in place of thymine. Sequences in option B, D and E does not resemble either of sequences, as given in question; options B, D and E are incorrect.