Question

Which one of the following techniques is used for the detection of proteins ?

• A. Nothern blotting
• B. Western blotting
• C. Southern blotting
• D. In-situ hybridization

Answer 1

Answer: (B)

Western blotting

Western blotting is the technique used for the detection of proteins. Separation of proteins is done using gel electrophoresis that takes place based on their molecular weight. These proteins are then transferred to a membrane in which a band is formed for every protein. In the final step, the membrane is incubated with labelled antibodies. The antibody is chosen such that it binds to the protein of interest. Thus, the correct answer is option B.