Question

Which palindromic base sequence is cut at the middle by a restriction enzyme

• A. $\frac{5^{\prime }-CGTTCG-3^{\prime }}{3^{\prime }-CCAAGC-5^{\prime }}$
• B. $\frac{5^{\prime }-GAATTC-3^{\prime }}{3^{\prime }-CTTAAG-5^{\prime }}$
• C. $\frac{5^{\prime }-CTACTG-3^{\prime }}{3^{\prime }-GTGCAA-5^{\prime }}$
• D. $\frac{3^{\prime }-CGAATG-5^{\prime }}{5^{\prime }-CGAATG-3^{\prime }}$

Answer 1

Answer: (B)

$\frac{5^{\prime }-GAATTC-3^{\prime }}{3^{\prime }-CTTAAG-5^{\prime }}$

A palindromic sequence is a sequence of DNA that is the exact same when read from 5' to 3' and from 3' to 5'. $\frac{5^{\prime }-GAATTC-3^{\prime }}{3^{\prime }-CTTAAG-5^{\prime }}$ In this DNA sequence the bases are same if the orientation kept same.

So the correct answer is option B.