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Question

Which part of the tobacco plant is infected by nematode Meloidogyne Incognita?


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Solution

Tobacco plants:

  1. Tobacco, Nicotiana tabacum, is an annual or perennial herbaceous plant in the Solanaceae family that is farmed for its leaves.
  2. The tobacco plant has a strong, hairy stem and broad, oval-shaped, simple leaves.
  3. Nicotine, an addictive substance that has both stimulant and depressive properties, is a component of tobacco.
  4. Tobacco leaves are used to create goods that can be smoked in pipes, cigars, or cigarettes, among other modes of consumption.

Infection of tobacco plant:

  1. Meloidogyne incognita infects the roots of tobacco plants.
  2. The RNAi technique is utilized to establish resistance to infection in these plants.
  3. RNA interference (RNAi) is a phenomenon induced by double-stranded RNA (dsRNA) in which gene expression is inhibited through specific degradation of mRNA.
  4. The process involves converting dsRNA into short RNAs that point ribonucleases toward target homologous mRNA.
  5. With the aid of an enzyme known as Dicer, long double-stranded RNA is cut up into manageable pieces. Small interfering RNA, or siRNA, is the name given to these tiny bits.
  6. The siRNAs are transported through the complex of RNA-induced silencing. The RNA is activated as the duplex unwinds. These complexes hinder translation while encouraging RNA breakdown.
  7. The siRNA bind to Argonaute protein and one of the strands from the double-stranded is removed.
  8. The mRNA target sequences are bound by the remaining strand. To control the target sequence, the Argonaute protein either cleaves the mRNA or uses the help of other agents.


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