Question

Which technique can be helpful in over-coming hybridisation barrier?

• A. Shoot tip culture
• B. Embryo rescue
• C. Protoplast fusion
• D. Both B and C

Answer 1

Answer: (D)

Both B and C

Hybridization barrier means limited interbreeding between two populations which reduces the variability. Protoplast fusion and embryo rescue are the technique which allows the plant to overcome the hybridization barrier by mixing the genetic pool of two population. Protoplast fusion is a technique to get hybrid plants with desired characters. The protoplast obtained from the parent plants are fused together to form a hybrid cell which is cultured on nutrient media. Embryo rescue is a technique which nurtures the immature or weak embryo, thus allowing it the chance to survive. Plant embryos are multicellular structures that have the potential to develop into a new plant. It allows the breeding of incompatible interspecific and intergeneric species and helps to overcome the hybridisation barrier.

So, the correct answer is option D.