Question

Which technique made it possible to genetically engineer living organisms?

• A. Recombinant DNA techniques
• B. Hybridisation
• C. X-ray diffraction
• D. Heavy isotope labelling

Answer 1

The correct option is A Recombinant DNA techniques

Recombinant DNA technology
Recombinant DNA technology allows the insertion of a piece of DNA into the host DNA and hence it has made it possible to genetically engineer living organisms. In this method, the recombinant DNA is transferred to a host cell to produce the proteins coded by the foreign DNA and a desired character is generated.

Heavy isotope labelling
Heavy isotopic labeling is a technique used to track the passage of a heavy isotope through a reaction or metabolic pathway. In this method, the reactant is 'labeled' by replacing specific atoms by their heavy isotope. The reactant is then allowed to undergo the reaction for easy detection.

X-ray diffraction
X-ray diffraction method is used to identify the atomic or molecular structure of a crystal, on the basis of diffraction pattern created by X-rays when it falls on the surface of the crystal

Hybridisation
Hybridisation of DNA takes place by combining two complementary single strands of DNA by the formation of hydrogen bonds. This technique is used in PCR and Southern blotting.

Final answer
(A) Recombinant DNA techniques